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calponin homology (ch) domain  (InterPro Inc)

 
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    InterPro Inc calponin homology (ch) domain
    Calponin Homology (Ch) Domain, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calponin+homology+(ch)+domain/pleckstrin+homology+like+domain/pm28188115-143-8-12
    Average 90 stars, based on 1 article reviews
    calponin homology (ch) domain - by Bioz Stars, 2026-09
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    Article Title: Differential mantle transcriptomics and characterization of growth-related genes in the diploid and triploid pearl oyster Pinctada fucata.
    Article Snippet: Article history: Received 23 June 2016 Received in revised form 5 January 2017 Accepted 6 January 2017 Available online xxxx To explore the molecular mechanism of triploidy effect in the pearl oyster Pinctada fucata, two RNA-seq libraries were constructed from themantle tissue of diploids and triploids by Roche-454massive parallel pyrosequencing.. The identification of differential expressed genes (DEGs) between diploid and triploid may reveal the molecular mechanism of triploidy effect.. In this study, 230 down-regulated and 259 up-regulated DEGs were obtained by comparison between diploid and triploid libraries.

    Article Title: Small-GTPase-Associated Signaling by the Guanine Nucleotide Exchange Factors CpDock180 and CpCdc24, the GTPase Effector CpSte20, and the Scaffold Protein CpBem1 in Claviceps purpurea
    Article Snippet: The resulting protein consists of 1,020 amino acids and contains the following InterPro domains: a DH-PH tandem domain characteristic for DH-GEFs, an N-terminal calponin homology (CH) domain, and a C-terminal PB1 domain ( ).



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    calponin homology (ch) domain  (Baines Food Consultancy)
    90
    Baines Food Consultancy calponin homology (ch) domain
    Time course analysis of larval brains at 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr e00176 , patr sk8 /patr e00176 , and patr sk1 /patr EY05252 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (A). 36 h ALH, Control, 6.5%, n = 11 BL; patr sk1 /patr e00176 , 38.4%, n = 11 BL, patr sk8 /patr e00176 , 67.4%, n = 10 BL and patr sk1 /patr EY05252 , 65.7%, n = 9 BL. 48 h ALH, Control, 0.9%, n = 10 BL; patr sk1 /patr e00176 , 29.3%, n = 11 BL, patr sk8 /patr e00176 , 65.5%, n = 10 BL and patr sk1 /patr EY05252 , 61.1%, n = 9 BL. 72 h ALH, Control, 0%, n = 12 BL; patr sk1 /patr e00176 , 11.5%, n = 8 BL and patr sk8 /patr e00176 , 31.1%, n = 8 BL. 96 h ALH, Control, 0%, n = 10 BL; patr sk1 /patr e00176 , 0%, n = 10 BL and patr sk8 /patr e00176 , 1.3%, n = 9 BL. Tabled analysis of EdU time‐course experiments of larval brains at 24 h, 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr sk8 , patr sk1 /patr EY05252 , patr sk1 /patr e00176 , and patr sk8 /patr e00176 . A schematic representation of full‐length and <t>truncated</t> <t>Patronin</t> domains. Patronin contains multiple domains: a <t>calponin</t> homology (CH) domain at the N‐terminus, three coiled‐coil (CC) repeats at the central region, and a signature CKK domain at the C‐terminus. Larval brains at 6 h ALH from control ( grh ‐Gal4/ UAS‐β‐Gal RNAi), UAS‐Patronin and UAS‐Patronin‐Venus controlled under grh ‐Gal4 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (E). Control, 28%, n = 10 BL; UAS‐Patronin , 26.7%, n = 9 BL and UAS‐Patronin‐Venus , 26.4%, n = 9 BL. Larval brains at 24 h ALH from the control ( grh ‐Gal4, UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG), and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8‐GFP; UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG) and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 13), patr RNAi I (#18462 Ra‐1, NIG; n = 15 quiescent NSCs), and patr RNAi II (VDRC 108927KK; n = 15 quiescent NSCs) in central brain (CB) at 16 h ALH. Larval brains at 24 h ALH from the control ( yw ) and patr sk1 /patr sk8 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8 GFP) and patr sk1 /patr sk8 driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn, and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 20 quiescent NSCs) and patr sk1 /patr sk8 ( n = 32 quiescent NSCs) in central brain (CB) at 16 h ALH. Data information: EdU incorporation was analyzed at 6 h ALH by feeding larvae at 2 h ALH with food supplemented with 0.2 mM EdU for 4 h for (E‐F) and at 36 h/48 h/72 h/ 96 h ALH by feeding larvae at 32 h/44 h/68 h/92 h ALH with food supplemented with 0.2 mM EdU for 4 h for (A‐C). White arrowheads point to NSCs without EdU incorporation (A). Data are presented as mean ± SD. In (B and F), statistical significance was determined by one‐way ANOVA with multiple comparisons. Ns, non‐significant, **** P < 0.0001. Scale bars: 5 μm for single quiescent NSC (H and K) and 10 μm for whole BL (A, E, G, and J). All replicates were biological. Source data are available online for this figure.
    Calponin Homology (Ch) Domain, supplied by Baines Food Consultancy, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calponin+homology+(ch)+domain/calponin+homology++ch++domain/pmc10481672-61-3-35
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    calponin homology (ch) domain  (InterPro Inc)
    90
    InterPro Inc calponin homology (ch) domain
    Time course analysis of larval brains at 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr e00176 , patr sk8 /patr e00176 , and patr sk1 /patr EY05252 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (A). 36 h ALH, Control, 6.5%, n = 11 BL; patr sk1 /patr e00176 , 38.4%, n = 11 BL, patr sk8 /patr e00176 , 67.4%, n = 10 BL and patr sk1 /patr EY05252 , 65.7%, n = 9 BL. 48 h ALH, Control, 0.9%, n = 10 BL; patr sk1 /patr e00176 , 29.3%, n = 11 BL, patr sk8 /patr e00176 , 65.5%, n = 10 BL and patr sk1 /patr EY05252 , 61.1%, n = 9 BL. 72 h ALH, Control, 0%, n = 12 BL; patr sk1 /patr e00176 , 11.5%, n = 8 BL and patr sk8 /patr e00176 , 31.1%, n = 8 BL. 96 h ALH, Control, 0%, n = 10 BL; patr sk1 /patr e00176 , 0%, n = 10 BL and patr sk8 /patr e00176 , 1.3%, n = 9 BL. Tabled analysis of EdU time‐course experiments of larval brains at 24 h, 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr sk8 , patr sk1 /patr EY05252 , patr sk1 /patr e00176 , and patr sk8 /patr e00176 . A schematic representation of full‐length and <t>truncated</t> <t>Patronin</t> domains. Patronin contains multiple domains: a <t>calponin</t> homology (CH) domain at the N‐terminus, three coiled‐coil (CC) repeats at the central region, and a signature CKK domain at the C‐terminus. Larval brains at 6 h ALH from control ( grh ‐Gal4/ UAS‐β‐Gal RNAi), UAS‐Patronin and UAS‐Patronin‐Venus controlled under grh ‐Gal4 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (E). Control, 28%, n = 10 BL; UAS‐Patronin , 26.7%, n = 9 BL and UAS‐Patronin‐Venus , 26.4%, n = 9 BL. Larval brains at 24 h ALH from the control ( grh ‐Gal4, UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG), and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8‐GFP; UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG) and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 13), patr RNAi I (#18462 Ra‐1, NIG; n = 15 quiescent NSCs), and patr RNAi II (VDRC 108927KK; n = 15 quiescent NSCs) in central brain (CB) at 16 h ALH. Larval brains at 24 h ALH from the control ( yw ) and patr sk1 /patr sk8 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8 GFP) and patr sk1 /patr sk8 driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn, and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 20 quiescent NSCs) and patr sk1 /patr sk8 ( n = 32 quiescent NSCs) in central brain (CB) at 16 h ALH. Data information: EdU incorporation was analyzed at 6 h ALH by feeding larvae at 2 h ALH with food supplemented with 0.2 mM EdU for 4 h for (E‐F) and at 36 h/48 h/72 h/ 96 h ALH by feeding larvae at 32 h/44 h/68 h/92 h ALH with food supplemented with 0.2 mM EdU for 4 h for (A‐C). White arrowheads point to NSCs without EdU incorporation (A). Data are presented as mean ± SD. In (B and F), statistical significance was determined by one‐way ANOVA with multiple comparisons. Ns, non‐significant, **** P < 0.0001. Scale bars: 5 μm for single quiescent NSC (H and K) and 10 μm for whole BL (A, E, G, and J). All replicates were biological. Source data are available online for this figure.
    Calponin Homology (Ch) Domain, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calponin+homology+(ch)+domain/pleckstrin+homology+like+domain/pm28188115-143-8-12
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    n-terminal calponin homology (ch) domain  (InterPro Inc)
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    InterPro Inc n-terminal calponin homology (ch) domain
    Time course analysis of larval brains at 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr e00176 , patr sk8 /patr e00176 , and patr sk1 /patr EY05252 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (A). 36 h ALH, Control, 6.5%, n = 11 BL; patr sk1 /patr e00176 , 38.4%, n = 11 BL, patr sk8 /patr e00176 , 67.4%, n = 10 BL and patr sk1 /patr EY05252 , 65.7%, n = 9 BL. 48 h ALH, Control, 0.9%, n = 10 BL; patr sk1 /patr e00176 , 29.3%, n = 11 BL, patr sk8 /patr e00176 , 65.5%, n = 10 BL and patr sk1 /patr EY05252 , 61.1%, n = 9 BL. 72 h ALH, Control, 0%, n = 12 BL; patr sk1 /patr e00176 , 11.5%, n = 8 BL and patr sk8 /patr e00176 , 31.1%, n = 8 BL. 96 h ALH, Control, 0%, n = 10 BL; patr sk1 /patr e00176 , 0%, n = 10 BL and patr sk8 /patr e00176 , 1.3%, n = 9 BL. Tabled analysis of EdU time‐course experiments of larval brains at 24 h, 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr sk8 , patr sk1 /patr EY05252 , patr sk1 /patr e00176 , and patr sk8 /patr e00176 . A schematic representation of full‐length and <t>truncated</t> <t>Patronin</t> domains. Patronin contains multiple domains: a <t>calponin</t> homology (CH) domain at the N‐terminus, three coiled‐coil (CC) repeats at the central region, and a signature CKK domain at the C‐terminus. Larval brains at 6 h ALH from control ( grh ‐Gal4/ UAS‐β‐Gal RNAi), UAS‐Patronin and UAS‐Patronin‐Venus controlled under grh ‐Gal4 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (E). Control, 28%, n = 10 BL; UAS‐Patronin , 26.7%, n = 9 BL and UAS‐Patronin‐Venus , 26.4%, n = 9 BL. Larval brains at 24 h ALH from the control ( grh ‐Gal4, UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG), and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8‐GFP; UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG) and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 13), patr RNAi I (#18462 Ra‐1, NIG; n = 15 quiescent NSCs), and patr RNAi II (VDRC 108927KK; n = 15 quiescent NSCs) in central brain (CB) at 16 h ALH. Larval brains at 24 h ALH from the control ( yw ) and patr sk1 /patr sk8 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8 GFP) and patr sk1 /patr sk8 driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn, and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 20 quiescent NSCs) and patr sk1 /patr sk8 ( n = 32 quiescent NSCs) in central brain (CB) at 16 h ALH. Data information: EdU incorporation was analyzed at 6 h ALH by feeding larvae at 2 h ALH with food supplemented with 0.2 mM EdU for 4 h for (E‐F) and at 36 h/48 h/72 h/ 96 h ALH by feeding larvae at 32 h/44 h/68 h/92 h ALH with food supplemented with 0.2 mM EdU for 4 h for (A‐C). White arrowheads point to NSCs without EdU incorporation (A). Data are presented as mean ± SD. In (B and F), statistical significance was determined by one‐way ANOVA with multiple comparisons. Ns, non‐significant, **** P < 0.0001. Scale bars: 5 μm for single quiescent NSC (H and K) and 10 μm for whole BL (A, E, G, and J). All replicates were biological. Source data are available online for this figure.
    N Terminal Calponin Homology (Ch) Domain, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    calponin homology (ch) domain  (Gallus BioPharmaceuticals)
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    Gallus BioPharmaceuticals calponin homology (ch) domain
    Time course analysis of larval brains at 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr e00176 , patr sk8 /patr e00176 , and patr sk1 /patr EY05252 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (A). 36 h ALH, Control, 6.5%, n = 11 BL; patr sk1 /patr e00176 , 38.4%, n = 11 BL, patr sk8 /patr e00176 , 67.4%, n = 10 BL and patr sk1 /patr EY05252 , 65.7%, n = 9 BL. 48 h ALH, Control, 0.9%, n = 10 BL; patr sk1 /patr e00176 , 29.3%, n = 11 BL, patr sk8 /patr e00176 , 65.5%, n = 10 BL and patr sk1 /patr EY05252 , 61.1%, n = 9 BL. 72 h ALH, Control, 0%, n = 12 BL; patr sk1 /patr e00176 , 11.5%, n = 8 BL and patr sk8 /patr e00176 , 31.1%, n = 8 BL. 96 h ALH, Control, 0%, n = 10 BL; patr sk1 /patr e00176 , 0%, n = 10 BL and patr sk8 /patr e00176 , 1.3%, n = 9 BL. Tabled analysis of EdU time‐course experiments of larval brains at 24 h, 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr sk8 , patr sk1 /patr EY05252 , patr sk1 /patr e00176 , and patr sk8 /patr e00176 . A schematic representation of full‐length and <t>truncated</t> <t>Patronin</t> domains. Patronin contains multiple domains: a <t>calponin</t> homology (CH) domain at the N‐terminus, three coiled‐coil (CC) repeats at the central region, and a signature CKK domain at the C‐terminus. Larval brains at 6 h ALH from control ( grh ‐Gal4/ UAS‐β‐Gal RNAi), UAS‐Patronin and UAS‐Patronin‐Venus controlled under grh ‐Gal4 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (E). Control, 28%, n = 10 BL; UAS‐Patronin , 26.7%, n = 9 BL and UAS‐Patronin‐Venus , 26.4%, n = 9 BL. Larval brains at 24 h ALH from the control ( grh ‐Gal4, UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG), and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8‐GFP; UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG) and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 13), patr RNAi I (#18462 Ra‐1, NIG; n = 15 quiescent NSCs), and patr RNAi II (VDRC 108927KK; n = 15 quiescent NSCs) in central brain (CB) at 16 h ALH. Larval brains at 24 h ALH from the control ( yw ) and patr sk1 /patr sk8 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8 GFP) and patr sk1 /patr sk8 driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn, and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 20 quiescent NSCs) and patr sk1 /patr sk8 ( n = 32 quiescent NSCs) in central brain (CB) at 16 h ALH. Data information: EdU incorporation was analyzed at 6 h ALH by feeding larvae at 2 h ALH with food supplemented with 0.2 mM EdU for 4 h for (E‐F) and at 36 h/48 h/72 h/ 96 h ALH by feeding larvae at 32 h/44 h/68 h/92 h ALH with food supplemented with 0.2 mM EdU for 4 h for (A‐C). White arrowheads point to NSCs without EdU incorporation (A). Data are presented as mean ± SD. In (B and F), statistical significance was determined by one‐way ANOVA with multiple comparisons. Ns, non‐significant, **** P < 0.0001. Scale bars: 5 μm for single quiescent NSC (H and K) and 10 μm for whole BL (A, E, G, and J). All replicates were biological. Source data are available online for this figure.
    Calponin Homology (Ch) Domain, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calponin+homology+(ch)+domain/calponin+homology++ch++domain/pm19896981-115-61-47
    Average 90 stars, based on 1 article reviews
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    Time course analysis of larval brains at 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr e00176 , patr sk8 /patr e00176 , and patr sk1 /patr EY05252 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (A). 36 h ALH, Control, 6.5%, n = 11 BL; patr sk1 /patr e00176 , 38.4%, n = 11 BL, patr sk8 /patr e00176 , 67.4%, n = 10 BL and patr sk1 /patr EY05252 , 65.7%, n = 9 BL. 48 h ALH, Control, 0.9%, n = 10 BL; patr sk1 /patr e00176 , 29.3%, n = 11 BL, patr sk8 /patr e00176 , 65.5%, n = 10 BL and patr sk1 /patr EY05252 , 61.1%, n = 9 BL. 72 h ALH, Control, 0%, n = 12 BL; patr sk1 /patr e00176 , 11.5%, n = 8 BL and patr sk8 /patr e00176 , 31.1%, n = 8 BL. 96 h ALH, Control, 0%, n = 10 BL; patr sk1 /patr e00176 , 0%, n = 10 BL and patr sk8 /patr e00176 , 1.3%, n = 9 BL. Tabled analysis of EdU time‐course experiments of larval brains at 24 h, 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr sk8 , patr sk1 /patr EY05252 , patr sk1 /patr e00176 , and patr sk8 /patr e00176 . A schematic representation of full‐length and truncated Patronin domains. Patronin contains multiple domains: a calponin homology (CH) domain at the N‐terminus, three coiled‐coil (CC) repeats at the central region, and a signature CKK domain at the C‐terminus. Larval brains at 6 h ALH from control ( grh ‐Gal4/ UAS‐β‐Gal RNAi), UAS‐Patronin and UAS‐Patronin‐Venus controlled under grh ‐Gal4 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (E). Control, 28%, n = 10 BL; UAS‐Patronin , 26.7%, n = 9 BL and UAS‐Patronin‐Venus , 26.4%, n = 9 BL. Larval brains at 24 h ALH from the control ( grh ‐Gal4, UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG), and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8‐GFP; UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG) and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 13), patr RNAi I (#18462 Ra‐1, NIG; n = 15 quiescent NSCs), and patr RNAi II (VDRC 108927KK; n = 15 quiescent NSCs) in central brain (CB) at 16 h ALH. Larval brains at 24 h ALH from the control ( yw ) and patr sk1 /patr sk8 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8 GFP) and patr sk1 /patr sk8 driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn, and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 20 quiescent NSCs) and patr sk1 /patr sk8 ( n = 32 quiescent NSCs) in central brain (CB) at 16 h ALH. Data information: EdU incorporation was analyzed at 6 h ALH by feeding larvae at 2 h ALH with food supplemented with 0.2 mM EdU for 4 h for (E‐F) and at 36 h/48 h/72 h/ 96 h ALH by feeding larvae at 32 h/44 h/68 h/92 h ALH with food supplemented with 0.2 mM EdU for 4 h for (A‐C). White arrowheads point to NSCs without EdU incorporation (A). Data are presented as mean ± SD. In (B and F), statistical significance was determined by one‐way ANOVA with multiple comparisons. Ns, non‐significant, **** P < 0.0001. Scale bars: 5 μm for single quiescent NSC (H and K) and 10 μm for whole BL (A, E, G, and J). All replicates were biological. Source data are available online for this figure.

    Journal: EMBO Reports

    Article Title: Patronin/CAMSAP promotes reactivation and regeneration of Drosophila quiescent neural stem cells

    doi: 10.15252/embr.202256624

    Figure Lengend Snippet: Time course analysis of larval brains at 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr e00176 , patr sk8 /patr e00176 , and patr sk1 /patr EY05252 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (A). 36 h ALH, Control, 6.5%, n = 11 BL; patr sk1 /patr e00176 , 38.4%, n = 11 BL, patr sk8 /patr e00176 , 67.4%, n = 10 BL and patr sk1 /patr EY05252 , 65.7%, n = 9 BL. 48 h ALH, Control, 0.9%, n = 10 BL; patr sk1 /patr e00176 , 29.3%, n = 11 BL, patr sk8 /patr e00176 , 65.5%, n = 10 BL and patr sk1 /patr EY05252 , 61.1%, n = 9 BL. 72 h ALH, Control, 0%, n = 12 BL; patr sk1 /patr e00176 , 11.5%, n = 8 BL and patr sk8 /patr e00176 , 31.1%, n = 8 BL. 96 h ALH, Control, 0%, n = 10 BL; patr sk1 /patr e00176 , 0%, n = 10 BL and patr sk8 /patr e00176 , 1.3%, n = 9 BL. Tabled analysis of EdU time‐course experiments of larval brains at 24 h, 36 h, 48 h, 72 h, and 96 h ALH from control, patr sk1 /patr sk8 , patr sk1 /patr EY05252 , patr sk1 /patr e00176 , and patr sk8 /patr e00176 . A schematic representation of full‐length and truncated Patronin domains. Patronin contains multiple domains: a calponin homology (CH) domain at the N‐terminus, three coiled‐coil (CC) repeats at the central region, and a signature CKK domain at the C‐terminus. Larval brains at 6 h ALH from control ( grh ‐Gal4/ UAS‐β‐Gal RNAi), UAS‐Patronin and UAS‐Patronin‐Venus controlled under grh ‐Gal4 were analyzed for EdU incorporation. NSCs were marked by Dpn. Quantification graph of EdU‐positive NSCs per brain lobe for genotypes in (E). Control, 28%, n = 10 BL; UAS‐Patronin , 26.7%, n = 9 BL and UAS‐Patronin‐Venus , 26.4%, n = 9 BL. Larval brains at 24 h ALH from the control ( grh ‐Gal4, UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG), and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8‐GFP; UAS ‐Dcr2/ UAS‐β‐Gal RNAi), patr RNAi I (#18462 Ra‐1, NIG) and patr RNAi II (VDRC 108927KK) driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 13), patr RNAi I (#18462 Ra‐1, NIG; n = 15 quiescent NSCs), and patr RNAi II (VDRC 108927KK; n = 15 quiescent NSCs) in central brain (CB) at 16 h ALH. Larval brains at 24 h ALH from the control ( yw ) and patr sk1 /patr sk8 were labeled with antibodies against Patronin and Dpn. Images of single quiescent NSCs from larval brains at 16 h ALH from control ( UAS ‐mCD8 GFP) and patr sk1 /patr sk8 driven by grh‐ Gal4 were labeled with antibodies against Patronin, Dpn, and GFP. Quantification of Patronin intensity in the primary protrusion of quiescent NSCs in control ( n = 20 quiescent NSCs) and patr sk1 /patr sk8 ( n = 32 quiescent NSCs) in central brain (CB) at 16 h ALH. Data information: EdU incorporation was analyzed at 6 h ALH by feeding larvae at 2 h ALH with food supplemented with 0.2 mM EdU for 4 h for (E‐F) and at 36 h/48 h/72 h/ 96 h ALH by feeding larvae at 32 h/44 h/68 h/92 h ALH with food supplemented with 0.2 mM EdU for 4 h for (A‐C). White arrowheads point to NSCs without EdU incorporation (A). Data are presented as mean ± SD. In (B and F), statistical significance was determined by one‐way ANOVA with multiple comparisons. Ns, non‐significant, **** P < 0.0001. Scale bars: 5 μm for single quiescent NSC (H and K) and 10 μm for whole BL (A, E, G, and J). All replicates were biological. Source data are available online for this figure.

    Article Snippet: Patronin contains a calponin homology (CH) domain at its amino terminus, three predicted coiled‐coil (CC) domains at its central region, and a CAMSAP/KIAA1078/KIA1543 (CKK) domain, which is the microtubule‐binding domain, at its carboxyl‐terminus (Fig ; Baines et al , ).

    Techniques: Control, Labeling